Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
Fig1: Western blot analysis of HDAC9 on human HDAC9 recombinant protein using anti-HDAC9 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of HDAC9 on HEK293 (1) and HDAC9-hIgGFc transfected HEK293 (2) cell lysate using anti-HDAC9 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with HDAC9 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human HDAC9 recombinant protein, HDAC9-hIgGFc transfected HEK293 cell lysate, Hela.
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
