Helicases are enzymes that catalyze the separation of double stranded DNA or RNA by utilizing ATP. LGP2, also known as probable ATP-dependent RNA helicase DHX58, is a 678 amino acid protein belonging to the helicase family. LGP2 acts as a negative regulator of host innate immune defense against viruses by binding dsRNA produced during viral replication. The repressor domain of LGP2 binds to RIG-I, a signaling protein involved in host defenses against hepatitis C virus (HCV). By preventing RIG-I multimerization, LGP2 negatively regulates RIG-I-mediated signaling. Localized to the cytoplasm, LGP2 contains one helicase ATP-binding domain and one helicase C-terminal domain.
Fig1: Western blot analysis of DHX58 on human DHX58 recombinant protein using anti-DHX58 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DHX58 on HEK293 (1) and DHX58-hIgGFc transfected HEK293 (2) cell lysate using anti-DHX58 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with DHX58 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human DHX58 recombinant protein, DHX58-hIgGFc transfected HEK293 cell lysate, Hela.
Application Notes
WB:1:500-1:2,000 FC:1:50-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
