POMC (pro-opiomelanocortin), also known as corticotropin-lipotropin, is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific post-translational processing by prohormone convertases. POMC is cleaved into ten hormone chains named NPP, γ-MSH, ACTH, α-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin. Defects in the gene that encodes POMC are the cause of POMC deficiency, which is characterized by red hair and adrenal insufficiency. Mutations in the POMC gene have also been linked to susceptibility to obesity. ACTH, also known as corticotropin, is a 39 amino acid active peptide that stimulates the secretion of cortisol by the adrenal gland. CLIP is a 21 amino acid neuropeptide secreted by corticotrope cells of adenohypophysis.
Fig1: Western blot analysis of POMC on human POMC recombinant protein using anti-POMC antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of POMC on HEK293 (1) and POMC-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab3a antibody at 1/1,000 dilution.
Application
Fig3:ICC staining POMC (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Positive Control
Human POMC recombinant protein, POMC-hIgGFc transfected HEK293 cell lysate, Hela.
Application Notes
WB:1:500-1:2,000 ICC:1:50-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
