Epsin 1 (EPN1) is an endocytic accessory protein, with significant similarity to the Xenopus mitotic phosphoprotein MP90. Epsin 1 interacts with Eps15 (the 伪 subunit of the Clathrin adaptor AP2), Clathrin and other accessory proteins. The mitotic phosphorylation of these proteins may be one of the mechanisms by which the invagination of Clathrin-coated pits is blocked in mitosis. Both epsin and Eps15, like other cytosolic components of the synaptic vesicle endocytic machinery, undergo constitutive phosphorylation and depolarization-dependent dephosphorylation in nerve terminals. Epsin 1 also contributes to the mechanism of Clathrin-vesicle-dependent endocytosis. The human Epsin 1 protein contains an epsin N-terminal homology (ENTH) region and a single Clathrin-binding (LVDLD) motif. Epsin 1 localizes to the leading edge of a vesicular coated pit where the membrane is being actively bent.
Fig1: Western blot analysis of EPN1 on human EPN1 recombinant protein using anti-EPN1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of EPN1 on HEK293 (1) and EPN1-hIgGFc transfected HEK293 (2) cell lysate using anti-EPN1 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of A431 cells with EPN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human EPN1 recombinant protein, EPN1-hIgGFc transfected HEK293 cell lysate, A431.
Application Notes
WB:1:500-1:2,000 FC:1:50-1:100
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
