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| Product Name | Caspase-2 [SR44-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP 2 antibody CASP-2 antibody Casp2 antibody CASP2_HUMAN antibody Caspase 2 antibody Caspase 2 apoptosis related cysteine peptidase antibody Caspase-2 subunit p12 antibody Caspase2 antibody ICH 1 antibody ICH 1 protease antibody ICH 1L antibody ICH1 antibody ICH1 protease antibody ICH1L antibody NEDD-2 antibody NEDD2 antibody Neural precursor cell expressed developmentally down-regulated protein 2 antibody PPP1R57 antibody Protease ICH-1 antibody Protein phosphatase 1 regulatory subunit 57 antibody |
| Product description | Caspase-2 (Nedd2, ICH-1) is an aspartate-specific cysteine protease that is activated in response to various apoptotic stimuli. Caspase-2 is unique among the caspases in that it has features of both upstream caspases (long prodomain) and downstream caspases (DEXD substrate specificity). Caspase-2 is highly expressed in the brain during development, and is expressed at low levels in adult tissue. Specifically, caspase-2 localizes to the mitochondria, the Golgi, the cytoplasm, and the nucleus. Caspase-2 exists as two isoforms, caspase-2L and caspase-2S, which are produced by alternative splicing and differ in their N and C-termini. Caspase-2L acts as a positive regulator of apoptosis, whereas caspase-2S functions as a negative regulator of apoptosis. Following apoptotic stimuli, the caspase-2L precursor undergoes cleavage at Asp-153 to produce a fragment (p30). The p30 fragment undergoes further cleavage to generate a fragment containing amino acids 153-308 (p18) and a fragment containing amino acids 317-435 (p13 or p14). As apoptosis progresses, the p13 (p14) fragment can undergo further processing to yield a fragment containing amino acids 331-435 (p12). |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CASP 2 antibody CASP-2 antibody Casp2 antibody CASP2_HUMAN antibody Caspase 2 antibody Caspase 2 apoptosis related cysteine peptidase antibody Caspase-2 subunit p12 antibody Caspase2 antibody ICH 1 antibody ICH 1 protease antibody ICH 1L antibody ICH1 antibody ICH1 protease antibody ICH1L antibody NEDD-2 antibody NEDD2 antibody Neural precursor cell expressed developmentally down-regulated protein 2 antibody PPP1R57 antibody Protease ICH-1 antibody Protein phosphatase 1 regulatory subunit 57 antibody |
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| Molecular Weight(MW) | 48/30/12 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Membrane. |
Application
Fig1: Western blot analysis of Caspase-2 on 293 cell lysate using anti-Caspase-2 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Caspase-2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Caspase-2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Caspase-2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caspase-2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Jurkat cells with Caspase-2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | 293, A549, Hela, human lung cancer tissue, mouse kidney tissue, human kidney tissue, Jurkat. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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