| 存储条件 |
|---|
| Product Name | Cdk5 [SR39-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1–3, PITALRE and PITSLRE. Cdk5 is thought to be involved in the G1-S transition of the cell cycle and is highly expressed in mature neurons. Activity of Cdk5 increases significantly during neuronal differentiation. Cdk5 has been postulated to be a neurofilament or tau protein kinase, based on its ability to phosphorylate these proteins in vitro. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,IHC,IP,FC |
| WB:1:500-1:1,000 IHC:1:50-1:200FC:1:50-1:100 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Cdk 5 antibody Cdk5 antibody CDK5_HUMAN antibody Cell division protein kinase 5 antibody Crk6 antibody Cyclin dependent kinase 5 antibody Cyclin-dependent kinase 5 antibody Protein kinase CDK5 splicing antibody PSSALRE antibody Serine threonine protein kinase PSSALRE antibody Serine/threonine-protein kinase PSSALRE antibody Tau protein kinase II catalytic subunit antibody TPKII catalytic subunit antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell membrane, Perikaryon, Cell junction |
Application
Fig1: Western blot analysis of Cdk5 on Hela cell lysates using anti-Cdk5 antibody at 1/500 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cdk5 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cdk5 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Hela cells with Cdk5 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, mouse brain tissue, rat brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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