| 存储条件 |
|---|
Product Profile
| Product Name | E-Cadherin [A0-G11-2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Arc 1 antibody CADH1_HUMAN antibody Cadherin 1 antibody cadherin 1 type 1 E-cadherin antibody Cadherin1 antibody CAM 120/80 antibody CD 324 antibody CD324 antibody CD324 antigen antibody cdh1 antibody CDHE antibody E-Cad/CTF3 antibody E-cadherin antibody ECAD antibody Epithelial cadherin antibody epithelial calcium dependant adhesion protein antibody LCAM antibody Liver cell adhesion molecule antibody UVO antibody Uvomorulin antibody |
| Product description | E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function. Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This in turn may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer. |
| Immunogen | recombinant protein |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:200 IHC:1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
Target Information
| Alternative Names | Arc 1 antibody CADH1_HUMAN antibody Cadherin 1 antibody cadherin 1 type 1 E-cadherin antibody Cadherin1 antibody CAM 120/80 antibody CD 324 antibody CD324 antibody CD324 antigen antibody cdh1 antibody CDHE antibody E-Cad/CTF3 antibody E-cadherin antibody ECAD antibody Epithelial cadherin antibody epithelial calcium dependant adhesion protein antibody LCAM antibody Liver cell adhesion molecule antibody UVO antibody Uvomorulin antibody |
|---|---|
| Molecular Weight(MW) | 130 kDa |
| Cellular Localization | Cell membrane |
Database Links
| SwissProt ID | P09803 |
|---|
Application
Application
Fig1: Western blot analysis of E-cadherin on different cell lysates using anti- E-cadherin antibody at 1/1000 dilution. Positive control: Lane 1: A431 Lane 2: SW480
Application
Fig2: ICC staining E-Cadherin in A431 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using E-Cadherin antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using E-Cadherin antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of HeLa cells with E-Cadherin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody
| Positive Control | A431, SW480, MCF-7, Hela, human liver carcinoma tissue, human colon carcinoma tissue |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:200 IHC:1:200 FC:1:50-1:100 |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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