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Product Profile
| Product Name | PBR [SA90-03] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Benzodiazapine receptor (peripheral) antibody Benzodiazepine peripheral binding site antibody BPBS antibody BZRP antibody DBI antibody IBP antibody Isoquinoline carboxamide-binding protein antibody MBR antibody mDRC antibody Mitochondrial benzodiazepine receptor antibody PBR antibody PBS antibody Peripheral benzodiazepine receptor antibody Peripheral benzodiazepine receptor-related protein antibody Peripheral type benzodiazepine receptor antibody Peripheral-type benzodiazepine receptor antibody pk18 antibody PKBS antibody PTBR antibody Ptbzr antibody PTBZR02 antibody RATPTBZR02 antibody translocator protein (18kDa) antibody Translocator protein antibody Tspo antibody Tspo1 antibody TSPOA_HUMAN antibody |
| Product description | Mitochondrial peripheral-type benzodiazepine receptor (PBR) is an indispensable element of the steroidogenic machinery, where it mediates the delivery of cholesterol to the inner mitochondrial side chain cleavage cytochrome P-450 upon ligand activation. PBR is composed of three subunits, an isoquinoline binding site, a voltage-dependent anion channel and an adenine nucleotide carrier. PBR is genetically conserved from bacteria to humans and in humans is widely expressed in peripheral organs, whereas in the brain, it is sparse and located mainly in glial cells. Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits the Leydig cell steroidogenesis by affecting PBR mRNA stability, thus inhibiting PBR expression, cholesterol transport into the mitochondria and subsequent steroid formation. A cytoplasmic protein, PRAX-1 ( peripheral benzodiazepine receptor-associated protein 1), is found to specifically interact with PBR. The polypeptide diazepam binding inhibitor is an endogenous PBR ligand. PBR also binds Ro 5-4864 (4’-chlorodiazepam) and PK 11185 (an isoquinoline carboxamide derivative), but not clonazepam, and PBR regulates the cholesterol transport that results in decreased circulating corticosterone levels. |
| Immunogen | recombinant protein |
Key Feature
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
Target Information
| Alternative Names | Benzodiazapine receptor (peripheral) antibody Benzodiazepine peripheral binding site antibody BPBS antibody BZRP antibody DBI antibody IBP antibody Isoquinoline carboxamide-binding protein antibody MBR antibody mDRC antibody Mitochondrial benzodiazepine receptor antibody PBR antibody PBS antibody Peripheral benzodiazepine receptor antibody Peripheral benzodiazepine receptor-related protein antibody Peripheral type benzodiazepine receptor antibody Peripheral-type benzodiazepine receptor antibody pk18 antibody PKBS antibody PTBR antibody Ptbzr antibody PTBZR02 antibody RATPTBZR02 antibody translocator protein (18kDa) antibody Translocator protein antibody Tspo antibody Tspo1 antibody TSPOA_HUMAN antibody |
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| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Mitochondrion membrane |
Database Links
| SwissProt ID | P30536P50637 |
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Application
Application
Fig1: Western blot analysis of PBR on different cell lysates using anti-PBR antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: NIH/3T3 Lane 3: HepG2
Application
Fig2: ICC staining PBR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PBR in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining PBR in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBR antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PBR antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PBR antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PBR antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with PBR antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Positive Control | PC-3M, MCF-7, Hela, HepG2, SW480, NIH/3T3, mouse kidney tissue, human colon cancer tissue, human kidney tissue, mouse testis tissue. |
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| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 |
Additional Information
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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