| 存储条件 |
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Product Profile
| Product Name | β-tubulin [1-B11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Beta 4 tubulin antibody Beta 5 tubulin antibody BetaTubulin antibody TBB5_HUMAN antibody TUBB antibody TUBB2 antibody TUBB2A antibody TUBB5 antibody tubulin beta 2A antibody Tubulin beta chain antibody Tubulin beta-5 chain antibody |
| Product description | Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP. |
| Immunogen | peptide |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:5,000-1:10,000 ICC:1:200 IHC:1:20 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 2mg/ml |
Target Information
| Alternative Names | Beta 4 tubulin antibody Beta 5 tubulin antibody BetaTubulin antibody TBB5_HUMAN antibody TUBB antibody TUBB2 antibody TUBB2A antibody TUBB5 antibody tubulin beta 2A antibody Tubulin beta chain antibody Tubulin beta-5 chain antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm ,cytoskeleton |
Database Links
| SwissProt ID | P07437 |
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Application
Application
Fig1: Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution. Positive control: Lane 1: NCCIT Lane 2: NIH/3T3 Lane 3: PC12 Lane 4: Mouse heart Lane 5: F9 Lane 6: zebrafish brain Lane 7: Hela
Application
Fig2: ICC staining β-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC stainingβ-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC stainingβ-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HeLa?cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Application
Fig7: Western blot analysis of β-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-β-tubulin antibody at 1/500 dilution.
| Positive Control | NCCIT, NIH/3T3, PC12, Mouse heart, F9, zebrafish brain, Hela HepG2, Mouse brain |
|---|---|
| Application Notes | WB:1:5,000-1:10,000 ICC:1:200 IHC:1:20 FC:1:50-1:100 |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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