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Product Profile
| Product Name | Goat Anti-Rabbit IgG H&L (TRITC) |
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| Antibody Type | Secondary Antibodies |
| Product description | This product is goat anti-rabbit IgG secondary antibody labeled NHS-Rhodamine, can be used in ICC and FC. |
| Immunogen | Rabbit IgG |
| Conjugation | TRITC |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFFC |
| ICC/IF:1:50-1:200 FC:1:50-1:100: | |
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
Application
Application
Fig1: ICC staining Fascin(ET1705-18)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&L (HA1016) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.
Application
Fig2: ICC staining SMC3(ET1703-35)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&L (HA1016) (1/100)as used as the secondary antibody. The result showed nuclear staining on Hela cells.
Application
Fig3: IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized human spleen tissue labeling CD34 with (ET1606-11) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (TRITC) (HA1016) secondary antibody at 1/200 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1016 at 1/200 dilution.
Application
Fig4: Flow cytometric analysis of Hela cells with SMC3 (ET1703-35) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1016 at 1/100 dilution.
| Application Notes | ICC/IF:1:50-1:200 FC:1:50-1:100: |
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Additional Information
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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