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Product Profile
| Product Name | Alpaca anti-rabbit IgG-HRP |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Higher resolution imaging due to the Alpaca anti-rabbit IgG FC, recombinant VHHs’ 10 times smaller size than conventional secondary antibodies. The small size enables better tissue penetration, antigen access and decreases the distance between the epitope and label. |
| Conjugation | HRP |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Camel |
| Tested Applications | ELISAIHCIPWB |
| IP: Use at an assay dependent concentration. ELISA: 1/5,000-1/20,000 IHC: 1/100-1/500 WB: 1/10,000-1/100,000: | |
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Application
Western blot
Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10
IHC
Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature. Alpaca anti-rabbit IgG FC, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.
| Application Notes | IP: Use at an assay dependent concentration. ELISA: 1/5,000-1/20,000 IHC: 1/100-1/500 WB: 1/10,000-1/100,000: |
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Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4), 0.5%BSA, 50%Glycerol. |
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